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human neuroblastoma cell line be2c  (ATCC)


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    ATCC human neuroblastoma cell line be2c
    Macrophages cultured with conditioned media from LMO1-overexpressing <t>BE2C</t> cells accelerate tumor-cell migration in vivo (A) Schematic diagram of experimental setup. U937 human monocytes were first differentiated to M0 macrophages with phorbol 12-myristate 13-acetate (PMA) and then cultured in conditioned media (CM) derived from control (“Ctl”) or LMO1-overexpressing (“LMO1”) BE2C cells. Ctl-CM-treated and LMO1-CM-treated U937 cells were labeled with ViaFluor 405 (green-fluorescent) dye. Each group of 405-labeled U937 cells was then combined with mCherry-labeled BE2C cells and co-injected into 2-day-old zebrafish through the perivitelline space (PVS, see diagram for the location of injection). (B–I) Ventral-view (B-E) or lateral-view (F-I) fluorescent images of transplanted zebrafish larvae at 1 day post-injection (dpi, left panels) or 3–4 dpi (right panels) (scale bars, 100 μm). Blue double arrowheads point to the site of injection in panels B-E. Black arrows point to U937 macrophages. Red arrows point to BE2C NB cancer cells. (J) Quantification of transplanted fish with NB cell migration ( n = 55). Migration of NB cells in transplanted recipients was defined as at least one or more mCherry-positive tumor cells located in the anterior regions of the fish, distant from the site of injection (outside of PVS and yolk). ∗∗∗∗ p ≤ 0.0001.
    Human Neuroblastoma Cell Line Be2c, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 458 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+neuroblastoma+cell+line+be2c/BE(2)-C/pmc12997303-218-1-6
    Average 96 stars, based on 458 article reviews
    human neuroblastoma cell line be2c - by Bioz Stars, 2026-09
    96/100 stars

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    1) Product Images from "LMO1 expression in neuroblastoma cells reprograms tumor-associated macrophages to promote metastasis"

    Article Title: LMO1 expression in neuroblastoma cells reprograms tumor-associated macrophages to promote metastasis

    Journal: iScience

    doi: 10.1016/j.isci.2026.115144

    Macrophages cultured with conditioned media from LMO1-overexpressing BE2C cells accelerate tumor-cell migration in vivo (A) Schematic diagram of experimental setup. U937 human monocytes were first differentiated to M0 macrophages with phorbol 12-myristate 13-acetate (PMA) and then cultured in conditioned media (CM) derived from control (“Ctl”) or LMO1-overexpressing (“LMO1”) BE2C cells. Ctl-CM-treated and LMO1-CM-treated U937 cells were labeled with ViaFluor 405 (green-fluorescent) dye. Each group of 405-labeled U937 cells was then combined with mCherry-labeled BE2C cells and co-injected into 2-day-old zebrafish through the perivitelline space (PVS, see diagram for the location of injection). (B–I) Ventral-view (B-E) or lateral-view (F-I) fluorescent images of transplanted zebrafish larvae at 1 day post-injection (dpi, left panels) or 3–4 dpi (right panels) (scale bars, 100 μm). Blue double arrowheads point to the site of injection in panels B-E. Black arrows point to U937 macrophages. Red arrows point to BE2C NB cancer cells. (J) Quantification of transplanted fish with NB cell migration ( n = 55). Migration of NB cells in transplanted recipients was defined as at least one or more mCherry-positive tumor cells located in the anterior regions of the fish, distant from the site of injection (outside of PVS and yolk). ∗∗∗∗ p ≤ 0.0001.
    Figure Legend Snippet: Macrophages cultured with conditioned media from LMO1-overexpressing BE2C cells accelerate tumor-cell migration in vivo (A) Schematic diagram of experimental setup. U937 human monocytes were first differentiated to M0 macrophages with phorbol 12-myristate 13-acetate (PMA) and then cultured in conditioned media (CM) derived from control (“Ctl”) or LMO1-overexpressing (“LMO1”) BE2C cells. Ctl-CM-treated and LMO1-CM-treated U937 cells were labeled with ViaFluor 405 (green-fluorescent) dye. Each group of 405-labeled U937 cells was then combined with mCherry-labeled BE2C cells and co-injected into 2-day-old zebrafish through the perivitelline space (PVS, see diagram for the location of injection). (B–I) Ventral-view (B-E) or lateral-view (F-I) fluorescent images of transplanted zebrafish larvae at 1 day post-injection (dpi, left panels) or 3–4 dpi (right panels) (scale bars, 100 μm). Blue double arrowheads point to the site of injection in panels B-E. Black arrows point to U937 macrophages. Red arrows point to BE2C NB cancer cells. (J) Quantification of transplanted fish with NB cell migration ( n = 55). Migration of NB cells in transplanted recipients was defined as at least one or more mCherry-positive tumor cells located in the anterior regions of the fish, distant from the site of injection (outside of PVS and yolk). ∗∗∗∗ p ≤ 0.0001.

    Techniques Used: Cell Culture, Migration, In Vivo, Derivative Assay, Control, Labeling, Injection

    Increased levels of metastasis-promoting cytokines are detected in the CM derived from LMO1-overexpressing BE2C cells (A) Cytokine arrays were probed using CM from Ctl BE2C cells (left) and LMO1-overexpressing BE2C cells (right) ( n = 2). Metastasis-promoting cytokines are labeled as follows: red boxes - Angiogenin; orange boxes - angiopoietin-2; green boxes - CD14; light-blue boxes - EGF; dark-blue boxes - Endoglin/CD105; purple boxes - GDF-15; pink boxes - HGF; gray boxes - ICAM-1/CD54; black boxes - CCL2/MCP-1; neon-green boxes - CXCL12/SDF-1 alpha; yellow boxes - VEGFA. (B) Quantitation of relative cytokine levels based on the normalized signal intensity on two independent cytokine array blots ( n = 2). (C) Relative expression of genes (determined by qRT-PCR) encoding the indicated cytokines in Ctl and LMO1-overexpressing BE2C cells ( n = 4). Data in (B) and (C) are presented as mean ± SD. ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001, and ∗∗∗∗ p ≤ 0.0001. ns; not significant.
    Figure Legend Snippet: Increased levels of metastasis-promoting cytokines are detected in the CM derived from LMO1-overexpressing BE2C cells (A) Cytokine arrays were probed using CM from Ctl BE2C cells (left) and LMO1-overexpressing BE2C cells (right) ( n = 2). Metastasis-promoting cytokines are labeled as follows: red boxes - Angiogenin; orange boxes - angiopoietin-2; green boxes - CD14; light-blue boxes - EGF; dark-blue boxes - Endoglin/CD105; purple boxes - GDF-15; pink boxes - HGF; gray boxes - ICAM-1/CD54; black boxes - CCL2/MCP-1; neon-green boxes - CXCL12/SDF-1 alpha; yellow boxes - VEGFA. (B) Quantitation of relative cytokine levels based on the normalized signal intensity on two independent cytokine array blots ( n = 2). (C) Relative expression of genes (determined by qRT-PCR) encoding the indicated cytokines in Ctl and LMO1-overexpressing BE2C cells ( n = 4). Data in (B) and (C) are presented as mean ± SD. ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001, and ∗∗∗∗ p ≤ 0.0001. ns; not significant.

    Techniques Used: Derivative Assay, Labeling, Quantitation Assay, Expressing, Quantitative RT-PCR

    Related Articles

    Cell Culture:

    Article Title: LMO1 expression in neuroblastoma cells reprograms tumor-associated macrophages to promote metastasis
    Article Snippet: The human neuroblastoma cell line BE2C (ATCC Cat# CRL-2268, RRID: CVCL_0529) and the U937 (ATCC Cat# CRL-1593.2, RRID:CVCL_0007) human monocytes were obtained from the American Type Culture Collection (ATCC).. All cell lines were authenticated using short tandem repeat (STR) profiling within the last three years and all experiments were performed with mycoplasma-free cells.All cell lines were authenticated using short tandem repeat (STR) profiling within the last three years and all experiments were performed with mycoplasma-free cells.

    Article Title: LMO1 expression in neuroblastoma cells reprograms tumor-associated macrophages to promote metastasis
    Article Snippet: The human neuroblastoma cell line BE2C (ATCC Cat# CRL-2268, RRID: CVCL_0529) and the U937 (ATCC Cat# CRL-1593.2, RRID:CVCL_0007) human monocytes were obtained from the American Type Culture Collection (ATCC).. All cell lines were authenticated using short tandem repeat (STR) profiling within the last three years and all experiments were performed with mycoplasmafree cells.All cell lines were authenticated using short tandem repeat (STR) profiling within the last three years and all experiments were performed with mycoplasmafree cells.

    Migration:

    Article Title: LMO1 expression in neuroblastoma cells reprograms tumor-associated macrophages to promote metastasis
    Article Snippet: The human neuroblastoma cell line BE2C (ATCC Cat# CRL-2268, RRID: CVCL_0529) and the U937 (ATCC Cat# CRL-1593.2, RRID:CVCL_0007) human monocytes were obtained from the American Type Culture Collection (ATCC).. All cell lines were authenticated using short tandem repeat (STR) profiling within the last three years and all experiments were performed with mycoplasma-free cells.All cell lines were authenticated using short tandem repeat (STR) profiling within the last three years and all experiments were performed with mycoplasma-free cells.

    Article Title: LMO1 expression in neuroblastoma cells reprograms tumor-associated macrophages to promote metastasis
    Article Snippet: The human neuroblastoma cell line BE2C (ATCC Cat# CRL-2268, RRID: CVCL_0529) and the U937 (ATCC Cat# CRL-1593.2, RRID:CVCL_0007) human monocytes were obtained from the American Type Culture Collection (ATCC).. All cell lines were authenticated using short tandem repeat (STR) profiling within the last three years and all experiments were performed with mycoplasmafree cells.All cell lines were authenticated using short tandem repeat (STR) profiling within the last three years and all experiments were performed with mycoplasmafree cells.

    In Vivo:

    Article Title: LMO1 expression in neuroblastoma cells reprograms tumor-associated macrophages to promote metastasis
    Article Snippet: The human neuroblastoma cell line BE2C (ATCC Cat# CRL-2268, RRID: CVCL_0529) and the U937 (ATCC Cat# CRL-1593.2, RRID:CVCL_0007) human monocytes were obtained from the American Type Culture Collection (ATCC).. All cell lines were authenticated using short tandem repeat (STR) profiling within the last three years and all experiments were performed with mycoplasma-free cells.All cell lines were authenticated using short tandem repeat (STR) profiling within the last three years and all experiments were performed with mycoplasma-free cells.

    Article Title: LMO1 expression in neuroblastoma cells reprograms tumor-associated macrophages to promote metastasis
    Article Snippet: The human neuroblastoma cell line BE2C (ATCC Cat# CRL-2268, RRID: CVCL_0529) and the U937 (ATCC Cat# CRL-1593.2, RRID:CVCL_0007) human monocytes were obtained from the American Type Culture Collection (ATCC).. All cell lines were authenticated using short tandem repeat (STR) profiling within the last three years and all experiments were performed with mycoplasmafree cells.All cell lines were authenticated using short tandem repeat (STR) profiling within the last three years and all experiments were performed with mycoplasmafree cells.

    Derivative Assay:

    Article Title: LMO1 expression in neuroblastoma cells reprograms tumor-associated macrophages to promote metastasis
    Article Snippet: The human neuroblastoma cell line BE2C (ATCC Cat# CRL-2268, RRID: CVCL_0529) and the U937 (ATCC Cat# CRL-1593.2, RRID:CVCL_0007) human monocytes were obtained from the American Type Culture Collection (ATCC).. All cell lines were authenticated using short tandem repeat (STR) profiling within the last three years and all experiments were performed with mycoplasma-free cells.All cell lines were authenticated using short tandem repeat (STR) profiling within the last three years and all experiments were performed with mycoplasma-free cells.

    Article Title: LMO1 expression in neuroblastoma cells reprograms tumor-associated macrophages to promote metastasis
    Article Snippet: The human neuroblastoma cell line BE2C (ATCC Cat# CRL-2268, RRID: CVCL_0529) and the U937 (ATCC Cat# CRL-1593.2, RRID:CVCL_0007) human monocytes were obtained from the American Type Culture Collection (ATCC).. All cell lines were authenticated using short tandem repeat (STR) profiling within the last three years and all experiments were performed with mycoplasmafree cells.All cell lines were authenticated using short tandem repeat (STR) profiling within the last three years and all experiments were performed with mycoplasmafree cells.

    Control:

    Article Title: LMO1 expression in neuroblastoma cells reprograms tumor-associated macrophages to promote metastasis
    Article Snippet: The human neuroblastoma cell line BE2C (ATCC Cat# CRL-2268, RRID: CVCL_0529) and the U937 (ATCC Cat# CRL-1593.2, RRID:CVCL_0007) human monocytes were obtained from the American Type Culture Collection (ATCC).. All cell lines were authenticated using short tandem repeat (STR) profiling within the last three years and all experiments were performed with mycoplasma-free cells.All cell lines were authenticated using short tandem repeat (STR) profiling within the last three years and all experiments were performed with mycoplasma-free cells.

    Article Title: LMO1 expression in neuroblastoma cells reprograms tumor-associated macrophages to promote metastasis
    Article Snippet: The human neuroblastoma cell line BE2C (ATCC Cat# CRL-2268, RRID: CVCL_0529) and the U937 (ATCC Cat# CRL-1593.2, RRID:CVCL_0007) human monocytes were obtained from the American Type Culture Collection (ATCC).. All cell lines were authenticated using short tandem repeat (STR) profiling within the last three years and all experiments were performed with mycoplasmafree cells.All cell lines were authenticated using short tandem repeat (STR) profiling within the last three years and all experiments were performed with mycoplasmafree cells.

    Labeling:

    Article Title: LMO1 expression in neuroblastoma cells reprograms tumor-associated macrophages to promote metastasis
    Article Snippet: The human neuroblastoma cell line BE2C (ATCC Cat# CRL-2268, RRID: CVCL_0529) and the U937 (ATCC Cat# CRL-1593.2, RRID:CVCL_0007) human monocytes were obtained from the American Type Culture Collection (ATCC).. All cell lines were authenticated using short tandem repeat (STR) profiling within the last three years and all experiments were performed with mycoplasma-free cells.All cell lines were authenticated using short tandem repeat (STR) profiling within the last three years and all experiments were performed with mycoplasma-free cells.

    Article Title: LMO1 expression in neuroblastoma cells reprograms tumor-associated macrophages to promote metastasis
    Article Snippet: The human neuroblastoma cell line BE2C (ATCC Cat# CRL-2268, RRID: CVCL_0529) and the U937 (ATCC Cat# CRL-1593.2, RRID:CVCL_0007) human monocytes were obtained from the American Type Culture Collection (ATCC).. All cell lines were authenticated using short tandem repeat (STR) profiling within the last three years and all experiments were performed with mycoplasmafree cells.All cell lines were authenticated using short tandem repeat (STR) profiling within the last three years and all experiments were performed with mycoplasmafree cells.

    Injection:

    Article Title: LMO1 expression in neuroblastoma cells reprograms tumor-associated macrophages to promote metastasis
    Article Snippet: The human neuroblastoma cell line BE2C (ATCC Cat# CRL-2268, RRID: CVCL_0529) and the U937 (ATCC Cat# CRL-1593.2, RRID:CVCL_0007) human monocytes were obtained from the American Type Culture Collection (ATCC).. All cell lines were authenticated using short tandem repeat (STR) profiling within the last three years and all experiments were performed with mycoplasma-free cells.All cell lines were authenticated using short tandem repeat (STR) profiling within the last three years and all experiments were performed with mycoplasma-free cells.

    Article Title: LMO1 expression in neuroblastoma cells reprograms tumor-associated macrophages to promote metastasis
    Article Snippet: The human neuroblastoma cell line BE2C (ATCC Cat# CRL-2268, RRID: CVCL_0529) and the U937 (ATCC Cat# CRL-1593.2, RRID:CVCL_0007) human monocytes were obtained from the American Type Culture Collection (ATCC).. All cell lines were authenticated using short tandem repeat (STR) profiling within the last three years and all experiments were performed with mycoplasmafree cells.All cell lines were authenticated using short tandem repeat (STR) profiling within the last three years and all experiments were performed with mycoplasmafree cells.

    Quantitation Assay:

    Article Title: LMO1 expression in neuroblastoma cells reprograms tumor-associated macrophages to promote metastasis
    Article Snippet: The human neuroblastoma cell line BE2C (ATCC Cat# CRL-2268, RRID: CVCL_0529) and the U937 (ATCC Cat# CRL-1593.2, RRID:CVCL_0007) human monocytes were obtained from the American Type Culture Collection (ATCC).. All cell lines were authenticated using short tandem repeat (STR) profiling within the last three years and all experiments were performed with mycoplasma-free cells.All cell lines were authenticated using short tandem repeat (STR) profiling within the last three years and all experiments were performed with mycoplasma-free cells.

    Article Title: LMO1 expression in neuroblastoma cells reprograms tumor-associated macrophages to promote metastasis
    Article Snippet: The human neuroblastoma cell line BE2C (ATCC Cat# CRL-2268, RRID: CVCL_0529) and the U937 (ATCC Cat# CRL-1593.2, RRID:CVCL_0007) human monocytes were obtained from the American Type Culture Collection (ATCC).. All cell lines were authenticated using short tandem repeat (STR) profiling within the last three years and all experiments were performed with mycoplasmafree cells.All cell lines were authenticated using short tandem repeat (STR) profiling within the last three years and all experiments were performed with mycoplasmafree cells.

    Expressing:

    Article Title: LMO1 expression in neuroblastoma cells reprograms tumor-associated macrophages to promote metastasis
    Article Snippet: The human neuroblastoma cell line BE2C (ATCC Cat# CRL-2268, RRID: CVCL_0529) and the U937 (ATCC Cat# CRL-1593.2, RRID:CVCL_0007) human monocytes were obtained from the American Type Culture Collection (ATCC).. All cell lines were authenticated using short tandem repeat (STR) profiling within the last three years and all experiments were performed with mycoplasma-free cells.All cell lines were authenticated using short tandem repeat (STR) profiling within the last three years and all experiments were performed with mycoplasma-free cells.

    Article Title: LMO1 expression in neuroblastoma cells reprograms tumor-associated macrophages to promote metastasis
    Article Snippet: The human neuroblastoma cell line BE2C (ATCC Cat# CRL-2268, RRID: CVCL_0529) and the U937 (ATCC Cat# CRL-1593.2, RRID:CVCL_0007) human monocytes were obtained from the American Type Culture Collection (ATCC).. All cell lines were authenticated using short tandem repeat (STR) profiling within the last three years and all experiments were performed with mycoplasmafree cells.All cell lines were authenticated using short tandem repeat (STR) profiling within the last three years and all experiments were performed with mycoplasmafree cells.

    Quantitative RT-PCR:

    Article Title: LMO1 expression in neuroblastoma cells reprograms tumor-associated macrophages to promote metastasis
    Article Snippet: The human neuroblastoma cell line BE2C (ATCC Cat# CRL-2268, RRID: CVCL_0529) and the U937 (ATCC Cat# CRL-1593.2, RRID:CVCL_0007) human monocytes were obtained from the American Type Culture Collection (ATCC).. All cell lines were authenticated using short tandem repeat (STR) profiling within the last three years and all experiments were performed with mycoplasma-free cells.All cell lines were authenticated using short tandem repeat (STR) profiling within the last three years and all experiments were performed with mycoplasma-free cells.

    Article Title: LMO1 expression in neuroblastoma cells reprograms tumor-associated macrophages to promote metastasis
    Article Snippet: The human neuroblastoma cell line BE2C (ATCC Cat# CRL-2268, RRID: CVCL_0529) and the U937 (ATCC Cat# CRL-1593.2, RRID:CVCL_0007) human monocytes were obtained from the American Type Culture Collection (ATCC).. All cell lines were authenticated using short tandem repeat (STR) profiling within the last three years and all experiments were performed with mycoplasmafree cells.All cell lines were authenticated using short tandem repeat (STR) profiling within the last three years and all experiments were performed with mycoplasmafree cells.



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    human neuroblastoma cell line be2c  (ATCC)
    96
    ATCC human neuroblastoma cell line be2c
    Macrophages cultured with conditioned media from LMO1-overexpressing <t>BE2C</t> cells accelerate tumor-cell migration in vivo (A) Schematic diagram of experimental setup. U937 human monocytes were first differentiated to M0 macrophages with phorbol 12-myristate 13-acetate (PMA) and then cultured in conditioned media (CM) derived from control (“Ctl”) or LMO1-overexpressing (“LMO1”) BE2C cells. Ctl-CM-treated and LMO1-CM-treated U937 cells were labeled with ViaFluor 405 (green-fluorescent) dye. Each group of 405-labeled U937 cells was then combined with mCherry-labeled BE2C cells and co-injected into 2-day-old zebrafish through the perivitelline space (PVS, see diagram for the location of injection). (B–I) Ventral-view (B-E) or lateral-view (F-I) fluorescent images of transplanted zebrafish larvae at 1 day post-injection (dpi, left panels) or 3–4 dpi (right panels) (scale bars, 100 μm). Blue double arrowheads point to the site of injection in panels B-E. Black arrows point to U937 macrophages. Red arrows point to BE2C NB cancer cells. (J) Quantification of transplanted fish with NB cell migration ( n = 55). Migration of NB cells in transplanted recipients was defined as at least one or more mCherry-positive tumor cells located in the anterior regions of the fish, distant from the site of injection (outside of PVS and yolk). ∗∗∗∗ p ≤ 0.0001.
    Human Neuroblastoma Cell Line Be2c, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+neuroblastoma+cell+line+be2c/BE(2)-C/pmc12997303-218-1-6
    Average 96 stars, based on 1 article reviews
    human neuroblastoma cell line be2c - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier

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    Macrophages cultured with conditioned media from LMO1-overexpressing BE2C cells accelerate tumor-cell migration in vivo (A) Schematic diagram of experimental setup. U937 human monocytes were first differentiated to M0 macrophages with phorbol 12-myristate 13-acetate (PMA) and then cultured in conditioned media (CM) derived from control (“Ctl”) or LMO1-overexpressing (“LMO1”) BE2C cells. Ctl-CM-treated and LMO1-CM-treated U937 cells were labeled with ViaFluor 405 (green-fluorescent) dye. Each group of 405-labeled U937 cells was then combined with mCherry-labeled BE2C cells and co-injected into 2-day-old zebrafish through the perivitelline space (PVS, see diagram for the location of injection). (B–I) Ventral-view (B-E) or lateral-view (F-I) fluorescent images of transplanted zebrafish larvae at 1 day post-injection (dpi, left panels) or 3–4 dpi (right panels) (scale bars, 100 μm). Blue double arrowheads point to the site of injection in panels B-E. Black arrows point to U937 macrophages. Red arrows point to BE2C NB cancer cells. (J) Quantification of transplanted fish with NB cell migration ( n = 55). Migration of NB cells in transplanted recipients was defined as at least one or more mCherry-positive tumor cells located in the anterior regions of the fish, distant from the site of injection (outside of PVS and yolk). ∗∗∗∗ p ≤ 0.0001.

    Journal: iScience

    Article Title: LMO1 expression in neuroblastoma cells reprograms tumor-associated macrophages to promote metastasis

    doi: 10.1016/j.isci.2026.115144

    Figure Lengend Snippet: Macrophages cultured with conditioned media from LMO1-overexpressing BE2C cells accelerate tumor-cell migration in vivo (A) Schematic diagram of experimental setup. U937 human monocytes were first differentiated to M0 macrophages with phorbol 12-myristate 13-acetate (PMA) and then cultured in conditioned media (CM) derived from control (“Ctl”) or LMO1-overexpressing (“LMO1”) BE2C cells. Ctl-CM-treated and LMO1-CM-treated U937 cells were labeled with ViaFluor 405 (green-fluorescent) dye. Each group of 405-labeled U937 cells was then combined with mCherry-labeled BE2C cells and co-injected into 2-day-old zebrafish through the perivitelline space (PVS, see diagram for the location of injection). (B–I) Ventral-view (B-E) or lateral-view (F-I) fluorescent images of transplanted zebrafish larvae at 1 day post-injection (dpi, left panels) or 3–4 dpi (right panels) (scale bars, 100 μm). Blue double arrowheads point to the site of injection in panels B-E. Black arrows point to U937 macrophages. Red arrows point to BE2C NB cancer cells. (J) Quantification of transplanted fish with NB cell migration ( n = 55). Migration of NB cells in transplanted recipients was defined as at least one or more mCherry-positive tumor cells located in the anterior regions of the fish, distant from the site of injection (outside of PVS and yolk). ∗∗∗∗ p ≤ 0.0001.

    Article Snippet: The human neuroblastoma cell line BE2C (ATCC Cat# CRL-2268, RRID: CVCL_0529) and the U937 (ATCC Cat# CRL-1593.2, RRID:CVCL_0007) human monocytes were obtained from the American Type Culture Collection (ATCC).

    Techniques: Cell Culture, Migration, In Vivo, Derivative Assay, Control, Labeling, Injection

    Increased levels of metastasis-promoting cytokines are detected in the CM derived from LMO1-overexpressing BE2C cells (A) Cytokine arrays were probed using CM from Ctl BE2C cells (left) and LMO1-overexpressing BE2C cells (right) ( n = 2). Metastasis-promoting cytokines are labeled as follows: red boxes - Angiogenin; orange boxes - angiopoietin-2; green boxes - CD14; light-blue boxes - EGF; dark-blue boxes - Endoglin/CD105; purple boxes - GDF-15; pink boxes - HGF; gray boxes - ICAM-1/CD54; black boxes - CCL2/MCP-1; neon-green boxes - CXCL12/SDF-1 alpha; yellow boxes - VEGFA. (B) Quantitation of relative cytokine levels based on the normalized signal intensity on two independent cytokine array blots ( n = 2). (C) Relative expression of genes (determined by qRT-PCR) encoding the indicated cytokines in Ctl and LMO1-overexpressing BE2C cells ( n = 4). Data in (B) and (C) are presented as mean ± SD. ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001, and ∗∗∗∗ p ≤ 0.0001. ns; not significant.

    Journal: iScience

    Article Title: LMO1 expression in neuroblastoma cells reprograms tumor-associated macrophages to promote metastasis

    doi: 10.1016/j.isci.2026.115144

    Figure Lengend Snippet: Increased levels of metastasis-promoting cytokines are detected in the CM derived from LMO1-overexpressing BE2C cells (A) Cytokine arrays were probed using CM from Ctl BE2C cells (left) and LMO1-overexpressing BE2C cells (right) ( n = 2). Metastasis-promoting cytokines are labeled as follows: red boxes - Angiogenin; orange boxes - angiopoietin-2; green boxes - CD14; light-blue boxes - EGF; dark-blue boxes - Endoglin/CD105; purple boxes - GDF-15; pink boxes - HGF; gray boxes - ICAM-1/CD54; black boxes - CCL2/MCP-1; neon-green boxes - CXCL12/SDF-1 alpha; yellow boxes - VEGFA. (B) Quantitation of relative cytokine levels based on the normalized signal intensity on two independent cytokine array blots ( n = 2). (C) Relative expression of genes (determined by qRT-PCR) encoding the indicated cytokines in Ctl and LMO1-overexpressing BE2C cells ( n = 4). Data in (B) and (C) are presented as mean ± SD. ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001, and ∗∗∗∗ p ≤ 0.0001. ns; not significant.

    Article Snippet: The human neuroblastoma cell line BE2C (ATCC Cat# CRL-2268, RRID: CVCL_0529) and the U937 (ATCC Cat# CRL-1593.2, RRID:CVCL_0007) human monocytes were obtained from the American Type Culture Collection (ATCC).

    Techniques: Derivative Assay, Labeling, Quantitation Assay, Expressing, Quantitative RT-PCR